Y-27632 dihydrochloride

Name: Y-27632 dihydrochloride
Brand: MedChemExpress
SKU: HY-10583G
Rating: 5.0 (246 reviews)

Cat. No.: HY-10583G: FAQs
Data Sheet Handling Instructions

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Y-27632 dihydrochloride (GMP) is the GMP level of Y-27632 dihydrochloride (HY-10583). GMP guidelines are used to produce Y-27632 dihydrochloride (GMP). GMP small molecules works appropriately as an auxiliary reagent for cell research manufacture. Y-27632 dihydrochloride is an orally active and ATP-competitive ROCK (Rho-kinase) inhibitor with antiepileptic effect.

For research use only. We do not sell to patients.

Y-27632 dihydrochloride Chemical Structure

CAS No. : 129830-38-2

Size	Price	Stock	Quantity
10 mg	USD 1280	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3600	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 5760	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 246 publication(s) in Google Scholar

Other Forms of Y-27632 dihydrochloride:

Y-27632 In-stock
Y-27632 dihydrochloride In-stock

242 Publications Citing Use of MCE Y-27632 dihydrochloride

: Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583. [Abstract]; Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).

: Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583. [Abstract]; Immunofluorescence imaging of LNCaP cells treated with the Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl). After the cells are cultured on different substrates for 3 days, inhibitors are added and incubated for 24 h.

: Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2018 Jun 28;37(1):128. [Abstract]; MHCC-97H and Huh-7 cells co-transfected with control or supervillin-specific siRNA and a RhoA(WT), RhoA(V14), or RhoA(N19) plasmids for 48 h are treated with PBS or Y27632 2HCl (10 μM) for 16 h during hypoxia, and then assayed for phosphorylated p38 and ERK by immunoblotting. β-actin is used as the loading control

: Y-27632 dihydrochloride purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Jul 31;8(47):82506-82530. [Abstract]; SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) cells were either untreated (-) or treated (+) with 10mM Y27632 over a period of 48 hr. SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) clones were either untreated (-) or treated (+) with 10 mM Y27632 followed by stimulation without (-) or with (+) 5 ng/mL of TGFβ1 for 48 hr.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Y-27632 dihydrochloride (GMP) (2 μM; 4-8 d) enhances the survival rate and inreases reprogramming of mouse fibroblasts directly converted into neuronal cells^[1].
Y-27632 dihydrochloride (GMP) induces human fibroblasts convert into functional cardiomyocytes^[2].
Y-27632 dihydrochloride (GMP) induces fibroblasts convert into pluripotent stem cells (hiPSCs)^[2].
Y-27632 dihydrochloride (GMP) (2-10 μM) promotes human EPS cell proliferation and induces primed hPSCs convert into hEPS cells^[3].
Y-27632 dihydrochloride (GMP) (10 μM) induces human EPS cell derivated from blastocysts^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

320.26

Formula

C₁₄H₂₃Cl₂N₃O

CAS No.

129830-38-2

Appearance

Powder

Color

White to off-white

SMILES

O=C([C@@H]1CC[C@]([C@H](N)C)([H])CC1)NC2=CC=NC=C2.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (312.25 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1225 mL	15.6123 mL	31.2246 mL
5 mM	0.6245 mL	3.1225 mL	6.2449 mL
10 mM	0.3122 mL	1.5612 mL	3.1225 mL

View the Complete Stock Solution Preparation Table

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Dosing volume
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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Li X, et al. Small-Molecule-Driven Direct Reprogramming of Mouse Fibroblasts into Functional Neurons. Cell Stem Cell. 2015 Aug 6;17(2):195-203. [Content Brief]

[2]. Cao N, et al. Conversion of human fibroblasts into functional cardiomyocytes by small molecules. Science. 2016 Jun 3;352(6290):1216-20. [Content Brief]

[3]. Choi IY, et al. Concordant but Varied Phenotypes among Duchenne Muscular Dystrophy Patient-Specific Myoblasts Derived using a Human iPSC-Based Model. Cell Rep. 2016 Jun 7;15(10):2301-2312. [Content Brief]

[4]. Yang Y, et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Cell. 2017 Apr 6;169(2):243-257.e25. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.1225 mL	15.6123 mL	31.2246 mL	78.0616 mL
	5 mM	0.6245 mL	3.1225 mL	6.2449 mL	15.6123 mL
	10 mM	0.3122 mL	1.5612 mL	3.1225 mL	7.8062 mL
	15 mM	0.2082 mL	1.0408 mL	2.0816 mL	5.2041 mL
	20 mM	0.1561 mL	0.7806 mL	1.5612 mL	3.9031 mL
	25 mM	0.1249 mL	0.6245 mL	1.2490 mL	3.1225 mL
	30 mM	0.1041 mL	0.5204 mL	1.0408 mL	2.6021 mL
	40 mM	0.0781 mL	0.3903 mL	0.7806 mL	1.9515 mL
	50 mM	0.0624 mL	0.3122 mL	0.6245 mL	1.5612 mL
	60 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3010 mL
	80 mM	0.0390 mL	0.1952 mL	0.3903 mL	0.9758 mL
	100 mM	0.0312 mL	0.1561 mL	0.3122 mL	0.7806 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.