COASY Protein, Human (His)

COASY protein is a bifunctional enzyme that plays a key role in catalyzing the fourth and fifth steps of the CoA biosynthetic pathway. The coaD domain promotes the phosphopantothenate adenylyltransferase activity of the fourth reaction, and the coaE domain drives the dephosphoCoA kinase activity of the fifth reaction. COASY Protein, Human (His) is the recombinant human-derived COASY protein, expressed by E. coli , with N-His labeled tag.

The COASY protein functions as a bifunctional enzyme, playing a pivotal role in catalyzing the fourth and fifth sequential steps of the CoA biosynthetic pathway. The phosphopantetheine adenylyltransferase activity, coded by the coaD domain, facilitates the fourth reaction, while the dephospho-CoA kinase activity, coded by the coaE domain, drives the fifth reaction in the pathway. This enzyme is integral to CoA biosynthesis regulation and may serve as a crucial point for controlling the overall process, ensuring the proper and efficient synthesis of CoA, a vital cofactor involved in various cellular metabolic pathways.

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is typically 15.42 ng/mL, corresponding to a specific activity is 64850.843 units/mg.

Human

E. coli

N-6*His

Q13057-1 (M296-D564)

80347  [NCBI]

MAINRFRLENDLEELALYQIQLLKDLRHTENEEDKVSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD

Approximately 32 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, pH 6.6, 8% trehalose, 8% mannitol and 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.