1. Neuronal Signaling
  2. Cholinesterase (ChE)
  3. Protopine hydrochloride

Protopine hydrochloride  (Synonyms: Corydinine hydrochloride)

Cat. No.: HY-N0793A
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Protopine hydrochloride, an isoquinoline alkaloid, is a specific reversible and competitive inhibitor of acetylcholinesterase. Protopine hydrochloride exhibits anti-inflammation, anti-microbial, anti-angiogenic and anti-tumour activity.

For research use only. We do not sell to patients.

Protopine hydrochloride Chemical Structure

Protopine hydrochloride Chemical Structure

CAS No. : 6164-47-2

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Description

Protopine hydrochloride, an isoquinoline alkaloid, is a specific reversible and competitive inhibitor of acetylcholinesterase. Protopine hydrochloride exhibits anti-inflammation, anti-microbial, anti-angiogenic and anti-tumour activity[1][2].

In Vitro

Protopine (10-40 μM, 24 h-96 h) hydrochloride inhibits cell viability, migration, invasion and EMT process of liver carcinoma cells (HepG2, Huh7)[2].
Protopine (10-40 μM, 24 h) hydrochloride induces apoptosis by increasing the expression of caspase-3 and caspase-9 in HepG2 and Huh7 cells, and inhibits the PI3K/Akt signaling pathway[2].
Protopine (10-40 μM, 6 h) hydrochloride induces generation of ROS in HepG2 and Huh7 cells[2].
Protopine (0-10 μg/mL) hydrochloride inhibits the uptake of serotonin transporter (SERT) in S6 cells and noradrenaline (NE) uptake in N1 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: HepG2, Huh7
Concentration: 10, 20, 40 μM
Incubation Time: 24 h
Result: Induced the cleavage of caspase-3 and caspase-9.
Decreased Bcl-2 and Bcl-xl level.
Induced the release of mitochondrial protein cytochrome c into the cytosol.
In Vivo

Protopine (0.1 and 1 mg/kg, i.p.) hydrochloride alleviates Scopolamine (HY-N0296) (1 mg/kg)-induced memory impairment in mice[1].
Protopine (5-20 mg/kg, i.v.) hydrochloride inhibits tumor growth and inhibits PI3K/Akt, and induces cleavage of caspase-3 in xenograft BALB/c mice (s.c. with HepG2 or Huh-7 cells)[2].
Protopine (5-20 mg/kg, i.p.) hydrochloride shows antidepressant-like effect in mice HTR and TST tests[3].
Protopine (1-4 mg/kg, i.p., once daily for 3 days) hydrochloride shows protective effect on the focal cerebral ischaemic induced injury in rats[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-hydroxy-DL-tryptophan (5-HTP)-induced mice model[3]
Dosage: 5, 10, 20 mg/kg
Administration: i.p.
Result: Increased the number of 5-HTP-induced head twitch response (HTR).
Decreased the immobility time tested in the Tail suspension test (TST).
Molecular Weight

389.83

Formula

C20H20ClNO5

CAS No.
SMILES

O=C1CC2=C(CN(CCC3=CC4=C(C=C31)OCO4)C)C5=C(C=C2)OCO5.Cl

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Protopine hydrochloride
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HY-N0793A
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