2. Immunology/Inflammation Vitamin D Related/Nuclear Receptor
  3. Glucocorticoid Receptor
  4. Methylprednisolone aceponate

Methylprednisolone aceponate  (Synonyms: ZK 91588; MPA)

Cat. No.: HY-103025 Purity: 98.85%
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Methylprednisolone aceponate (ZK 91588) is a glucocorticoid and anti-inflammatory agent with weak systemic effects. Methylprednisolone aceponate is a selective glucocorticoid receptor Ligand.Methylprednisolone aceponate can be used for research of eczema and other inflammatory skin disorders.

For research use only. We do not sell to patients.

Methylprednisolone aceponate Chemical Structure

Methylprednisolone aceponate Chemical Structure

CAS No. : 86401-95-8

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10 mg USD 170 In-stock
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Based on 1 publication(s) in Google Scholar

Methylprednisolone aceponate Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Methylprednisolone aceponate (ZK 91588) is a glucocorticoid and anti-inflammatory agent with weak systemic effects. Methylprednisolone aceponate is a selective glucocorticoid receptor Ligand.Methylprednisolone aceponate can be used for research of eczema and other inflammatory skin disorders[1][2][3].

In Vitro

Methylprednisolone aceponate inhibits collagenase promoter activity (in HeLa cells), LPS-induced IL-12p40 secretion (in human PBMCs) and phytohemagglutinin-induced IFN-γ secretion (in human PBMCs) with IC50s of 9.3, 16.8, 15.2 nM[3].
Methylprednisolone aceponate induces MMTV promoter and TAT activities with EC50s of 21.8 and 20.5 nM respectively[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methylprednisolone aceponate Related Antibodies
In Vivo

Methylprednisolone aceponate (topically applied, 50 μL,，Coroton oil-induced Evan blue edema) shows anti-inflammatory effect with an IC50 of 0.0015%, with low systemic side effect[1].
Methylprednisolone aceponate (0.0001%-0.1%, topically applied) inhibits oedema formation with ED50 of 0.002% in irritant contact dermatitis in mice and rat[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Irritant contact dermatitis mice and rat[3]
Dosage: 0.0001%-0.1%
Administration: Topically applied, 10 µL for mice and 20 µL for rats.
Result: Significantly inhibited ear inflammation.
Clinical Trial
Molecular Weight

472.57

Formula

C27H36O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(OCC([C@@]1([C@@]2([C@@]([C@@]3([H])[C@]([C@@]4(C([C@@H](C)C3)=CC(C=C4)=O)C)([H])[C@@H](O)C2)([H])CC1)C)OC(CC)=O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (211.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1161 mL 10.5804 mL 21.1609 mL
5 mM 0.4232 mL 2.1161 mL 4.2322 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1161 mL 10.5804 mL 21.1609 mL 52.9022 mL
5 mM 0.4232 mL 2.1161 mL 4.2322 mL 10.5804 mL
10 mM 0.2116 mL 1.0580 mL 2.1161 mL 5.2902 mL
15 mM 0.1411 mL 0.7054 mL 1.4107 mL 3.5268 mL
20 mM 0.1058 mL 0.5290 mL 1.0580 mL 2.6451 mL
25 mM 0.0846 mL 0.4232 mL 0.8464 mL 2.1161 mL
30 mM 0.0705 mL 0.3527 mL 0.7054 mL 1.7634 mL
40 mM 0.0529 mL 0.2645 mL 0.5290 mL 1.3226 mL
50 mM 0.0423 mL 0.2116 mL 0.4232 mL 1.0580 mL
60 mM 0.0353 mL 0.1763 mL 0.3527 mL 0.8817 mL
80 mM 0.0265 mL 0.1323 mL 0.2645 mL 0.6613 mL
100 mM 0.0212 mL 0.1058 mL 0.2116 mL 0.5290 mL
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Methylprednisolone aceponate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylprednisolone aceponate86401-95-8ZK 91588 MPAZK91588ZK-91588Glucocorticoid ReceptorDermatitisoedemaanti-inflammatoryglucocorticoideczemaedemaInhibitorinhibitorinhibit

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