Bunyavirus SFTSV NSs utilizes autophagy to escape the antiviral innate immune response

Severe fever with thrombocytopenia syndrome virus (SFTSV) nonstructural protein (NSs) is an important viral virulence factor that sequesters multiple Antiviral proteins into inclusion bodies to escape the Antiviral innate immune response. However, the mechanism of the NSs restricting host innate immunity remains largely elusive. Here, we found that the NSs induced complete macroautophagy/Autophagy by interacting with the CCD domain of BECN1, thereby promoting the formation of a BECN1-dependent Autophagy initiation complex. Importantly, our data showed that the NSs sequestered Antiviral proteins such as TBK1 into autophagic vesicles, and therefore promoted the degradation of TBK1 and other Antiviral proteins. In addition, the 8A mutant of NSs reduced the induction of BECN1-dependent Autophagy flux and degradation of Antiviral immune proteins. In conclusion, our results indicated that SFTSV NSs sequesters Antiviral proteins into autophagic vesicles for degradation and to escape Antiviral immune responses.

Autophagy; SFTSV; TBK1; immune escape; inclusion bodies.