2. Academic Validation
  3. Isolation and Characterization of a Novel Mammary Adenocarcinoma, MCa-P1362, with Hormone Receptor Expression, Human Epidermal Growth Factor Receptor 2 Positivity, and Enrichment in Cancer and Mesenchymal Stem Cells

Isolation and Characterization of a Novel Mammary Adenocarcinoma, MCa-P1362, with Hormone Receptor Expression, Human Epidermal Growth Factor Receptor 2 Positivity, and Enrichment in Cancer and Mesenchymal Stem Cells

  • Am J Pathol. 2024 Jun;194(6):1137-1153. doi: 10.1016/j.ajpath.2024.02.013.
Samir Jana 1,  Wende Li 2,  Pin-Ji Lei 2,  Zixiong Wang 1,  Shaye Kibara 1,  Peigen Huang 3,  Dennis Jones 4
Affiliations

Affiliations

  • 1 Department of Pathology and Laboratory Medicine, Boston University Chobanian and Avedisian School of Medicine, Boston, Massachusetts.
  • 2 Edwin L. Steele Laboratories for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
  • 3 Edwin L. Steele Laboratories for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts. Electronic address: [email protected].
  • 4 Department of Pathology and Laboratory Medicine, Boston University Chobanian and Avedisian School of Medicine, Boston, Massachusetts. Electronic address: [email protected].
PMID: 38749609 DOI: 10.1016/j.ajpath.2024.02.013
Abstract

Preclinical models that display spontaneous metastasis are necessary to improve the therapeutic options for hormone receptor-positive breast cancers. Within this study, detailed cellular and molecular characterization was conducted on MCa-P1362, a newly established mouse model of metastatic breast Cancer that is syngeneic in BALB/c mice. MCa-P1362 Cancer cells express Estrogen Receptor, Progesterone Receptor, and the human epidermal growth factor receptor 2. MCa-P1362 Cancer cells proliferate in vitro and in vivo in response to estrogen, yet do not depend on steroid Hormones for growth and tumor progression. Analysis of MCa-P1362 tumor explants revealed the tumors contained a mixture of Cancer cells and mesenchymal stromal cells. Through transcriptomic and functional analyses of both Cancer and stromal cells, stem cells were detected within both populations. Functional studies demonstrated that MCa-P1362 Cancer Stem Cells drove tumor initiation, whereas stromal cells from these tumors contributed to drug resistance. MCa-P1362 may serve as a useful preclinical model to investigate the cellular and molecular basis of breast tumor progression and therapeutic resistance.

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