2. Academic Validation
  3. SNHG1 functions as a ceRNA in hypertrophic scar fibroblast proliferation and apoptosis through miR-320b/CTNNB1 axis

SNHG1 functions as a ceRNA in hypertrophic scar fibroblast proliferation and apoptosis through miR-320b/CTNNB1 axis

  • Arch Dermatol Res. 2023 Feb 9. doi: 10.1007/s00403-022-02516-y.
Qiaoling Li 1,  Bowei Zhang 2,  Jie Lu 3,  Qingbiao Wa 3,  Mei He 3,  Lixia Xie 3,  Lei Zhang 3
Affiliations

Affiliations

  • 1 Center of Medical Cosmetology, Chengdu Second People's Hospital, No. 10, Qingyun South Street, Jinjiang District, Chengdu, 610011, Sichuan, China. [email protected].
  • 2 Department of Vascular and Thyroid Surgery, Sichuan Provincial People's Hospital, Sichuan Academy of Medical Sciences, Chengdu, 610072, Sichuan, China.
  • 3 Center of Medical Cosmetology, Chengdu Second People's Hospital, No. 10, Qingyun South Street, Jinjiang District, Chengdu, 610011, Sichuan, China.
PMID: 36754869 DOI: 10.1007/s00403-022-02516-y
Abstract

Hypertrophic scar (HS) is a fibrotic disease caused by skin injury. Competing endogenous RNA (ceRNA) has been demonstrated to implicate in the regulation of cell malignant phenotypes. This research aims to reveal the effect of catenin beta 1 (CTNNB1) on the functions of hypertrophic scar fibroblasts (HSFBs) and its role in a ceRNA network. RNA expression level was assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). The proliferation and Apoptosis of HSFB was detected via Cell Counting Kit-8 (CCK-8) assay and flow cytometry analysis. Mechanism experiments included RNA pull down assay, luciferase reporter assay and RNA-binding protein immunoprecipitation (RIP) assay were applied to analyze the upstream molecular mechanism of CTNNB1. CTNNB1 was highly expressed in HSFB. CTNNB1 depletion repressed malignant growth of HSFB. Mechanically, CTNNB1 was targeted by microRNA-320b (miR-320b) in HSFB. Small nucleolar RNA host gene 1 (SNHG1) aced as a ceRNA to upregulate CTNNB1 expression via sponging miR-320b in HSFB. CTNNB1 overexpression could reverse the impact of SNHG1 depletion on the proliferation and Apoptosis of HSFB. SNHG1 acts as a ceRNA in modulating HSFB proliferation and Apoptosis through miR-320b/CTNNB1 axis. SNHG1 act as a ceRNA to promote HSFB growth by sponging miR-320b to upregulate CTNNB1.

Keywords

CTNNB1; Hypertrophic scar fibroblast; Proliferation; SNHG1; miR-320b.

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