2. Academic Validation
  3. Antifungal activity of 2'-hydroxychalcone loaded in nanoemulsion against Paracoccidioides spp

Antifungal activity of 2'-hydroxychalcone loaded in nanoemulsion against Paracoccidioides spp

  • Future Microbiol. 2020 Jan:15:21-33. doi: 10.2217/fmb-2019-0095.
Kaila P Medina-Alarcón 1,  Junya de L Singulani 1,  Luiz A Dutra 2,  Nayla de S Pitangui 1,  Marcelo A Pereira-da-Silva 3 4,  Mariana B Dos Santos 5,  Gabriela M Ayusso 5,  Luis O Regasini 5,  Christiane P Soares 6,  Marlus Chorilli 7,  Maria Js Mendes-Giannini 1,  Ana M Fusco-Almeida 1
Affiliations

Affiliations

  • 1 Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquara, Proteomics Center, Mycology Laboratory, Araraquara, SP, Brazil.
  • 2 Structural Genomics Consortium, Research Institute-UNICAMP, Campinas, SP, Brasil.
  • 3 Institute of Physics of Sao Carlos, IFSC/USP, Sao Carlos, SP, Brazil.
  • 4 Paulista Central University Center, UNICEP, Sao Carlos, SP, Brazil.
  • 5 Universidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto, Department of Chemistry and Environmental Sciences, São José do Rio Preto, SP, Brasil.
  • 6 Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquara, Department of Clinical Analysis, Cytology Laboratory, Araraquara, SP, Brazil.
  • 7 Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquara, Department of Drugs and Medicines, Araraquara, SP, Brazil.
PMID: 32043361 DOI: 10.2217/fmb-2019-0095
Abstract

Aim: This study aimed to evaluate the activity of 2'-hydroxychalcone-loaded in nanoemulsion (NLS + 2'chalc), the cytotoxic effect and toxicity against Paracoccidioides brasiliensis and Paracoccidioides lutzii using a zebrafish model. Materials & methods: Preparation and physical-chemical characterization of nanoemulsion (NLS) and NLS + 2'chalc were performed. MIC and minimum fungicide concentration, cytotoxicity and toxicity were also evaluated in the Danio rerio model. Results: NLS + 2'chalc showed fungicidal activity against Paracoccidioides spp. without cytotoxicity in MRC5 and HepG2 lines. It also had high selectivity index values and no toxicity in the zebrafish model based on MIC values. Conclusion: NLS + 2'chalc is a potential new alternative treatment for paracoccidioidomycosis.

Keywords

Paracoccidioides spp.; antifungal; nanostructured lipid system; zebrafish model.

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