1. Academic Validation
  2. ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma

ISG20 serves as a potential biomarker and drives tumor progression in clear cell renal cell carcinoma

  • Aging (Albany NY). 2020 Jan 30;12(2):1808-1827. doi: 10.18632/aging.102714.
Tianbo Xu 1 Hailong Ruan 1 Su Gao 2 Jingchong Liu 1 Yuenan Liu 1 Zhengshuai Song 3 Qi Cao 1 Keshan Wang 1 Lin Bao 1 Di Liu 1 Junwei Tong 1 Jian Shi 1 Huageng Liang 1 Hongmei Yang 4 Ke Chen 1 Xiaoping Zhang 1
Affiliations

Affiliations

  • 1 Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
  • 2 Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
  • 3 Department of Urology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
  • 4 Department of Pathogenic Biology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan 430030, China.
Abstract

Clear cell renal cell carcinoma (ccRCC) is one of the most common malignancies and lacks reliable biomarkers for diagnosis and prognosis, which results in high incidence and mortality rates of ccRCC. In this study, ISG20, HJURP, and FOXM1 were identified as hub genes via weighted gene co-expression network analysis (WGCNA) and COX regression analysis. Samples validation showed that only ISG20 was up-regulated in ccRCC. Therefore, ISG20 was selected for further study. High ISG20 expression was associated with poor overall survival and disease-free survival. Furthermore, the expression of ISG20 could effectively differentiate ccRCC from normal tissues and was positively correlated to clinical stages. Functional experiments proved that knockdown of ISG20 expression could obviously inhibit cell growth, migration, and invasion in ccRCC cells. To find the potential mechanisms of ISG20, gene set enrichment analysis (GSEA) was performed and revealed that high expression of ISG20 was significantly involved in metastasis and cell cycle pathways. In addition, we found that ISG20 could regulate the expression of MMP9 and CCND1. In conclusion, these findings suggested that ISG20 promoted cell proliferation and metastasis via regulating MMP9/CCND1 expression and might serve as a potential biomarker and therapeutic target in ccRCC.

Keywords

ISG20; bioinformatics; biomarker; clear cell renal cell carcinoma; tumor progression.

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