2. Academic Validation
  3. Systematic identification of flavonols, flavonol glycosides, triterpene and siraitic acid glycosides from Siraitia grosvenorii using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combined with a screening strategy

Systematic identification of flavonols, flavonol glycosides, triterpene and siraitic acid glycosides from Siraitia grosvenorii using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combined with a screening strategy

  • J Pharm Biomed Anal. 2017 May 10:138:240-248. doi: 10.1016/j.jpba.2017.01.059.
Zhi-Xing Qing 1,  Huan Zhao 2,  Qi Tang 3,  Chang-Ming Mo 4,  Peng Huang 5,  Pi Cheng 6,  Peng Yang 7,  Xue-Yi Yang 8,  Xiu-Bin Liu 9,  Ya-Jie Zheng 10,  Jian-Guo Zeng 11
Affiliations

Affiliations

  • 1 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: [email protected].
  • 2 State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China. Electronic address: [email protected].
  • 3 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: [email protected].
  • 4 Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China. Electronic address: [email protected].
  • 5 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: [email protected].
  • 6 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: [email protected].
  • 7 College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: [email protected].
  • 8 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: [email protected].
  • 9 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: [email protected].
  • 10 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China. Electronic address: [email protected].
  • 11 National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resources and Initiative & Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, 410128, China; College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. Electronic address: [email protected].
PMID: 28226282 DOI: 10.1016/j.jpba.2017.01.059
Abstract

The fruits of Siraitia grosvenorii are considered to be health-promoting because of the diversity of their bioactive ingredients. In the present study, a screening method, using high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (HPLC-Q-TOF-MS) combined with a screening strategy, has been established. The technology was used to systematically screening the targeted metabolites, primarily from the complex matrix of S. grosvenorii. The compounds were then identified by their exact masses and characteristic fragment ions, in comparison with the fragmentation behaviors of 19 references. Finally, 122 compounds, including 53 Flavonols and flavonol glycosides, 59 triterpene glycosides and 10 siraitic acid glycosides, were screened and identified in 10-, 50- and 80-day fruits, roots, stems and leaves of S. grosvenorii. 98 of them were reported for the first time. Additionally, the distribution of all identified components in different parts of the plant was determined and metabolic networks for flavonol and triterpene glycosides were proposed.

Keywords

Flavonol glycosides; HPLC-Q-TOF-MS; Siraitia grosvenorii; Siraitic acid glycosides; Triterpene glycosides.

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