2. Academic Validation
  3. Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step

Preparative isolation and purification of phlorotannins from Ecklonia cava using centrifugal partition chromatography by one-step

  • Food Chem. 2014 Sep 1:158:433-7. doi: 10.1016/j.foodchem.2014.02.112.
Ji-Hyeok Lee 1,  Ju-Young Ko 1,  Jae-Young Oh 1,  Chul-Young Kim 2,  Hee-Ju Lee 3,  Jaeil Kim 4,  You-Jin Jeon 5
Affiliations

Affiliations

  • 1 Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
  • 2 Natural Product Research Center, Hanyang University, Daejeon-dong, Ahnsan, Gyeongi-do, Republic of Korea.
  • 3 Natural Product Research Center, KIST Gangneung Institute, Daejeon-dong, Gangneung, Gangwon-do, Republic of Korea.
  • 4 Department of Food Science and Technology, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: [email protected].
  • 5 Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: [email protected].
PMID: 24731366 DOI: 10.1016/j.foodchem.2014.02.112
Abstract

Various bioactive phlorotannins of Ecklonia cava (e.g., dieckol, eckol, 6,6-bieckol, phloroglucinol, phloroeckol, and phlorofucofuroeckol-A) are reported. However, their isolation and purification are not easy. Centrifugal partition chromatography (CPC) can be used to efficiently purify the various bioactive-compounds efficiently from E. cava. Phlorotannins are successfully isolated from the ethyl acetate (EtOAc) fraction of E. cava by CPC with a two-phase solvent system comprising n-hexane:EtOAc:methanol:water (2:7:3:7, v/v) solution. The dieckol (fraction I, 40.2mg), phlorofucofuroeckol-A (fraction III, 31.1mg), and fraction II (34.1mg) with 2,7-phloroglucinol-6,6-bieckol and pyrogallol-phloroglucinol-6,6-bieckol are isolated from the crude extract (500 mg) by a one-step CPC system. The purities of the isolated dieckol and phlorofucofuroeckol-A are ⩾90% according to high performance liquid chromatography (HPLC) and electrospray ionization multi stage tandem mass spectrometry analyses. The purified 2,7-phloroglucinol-6,6-bieckol and pyrogallol-phloroglucinol-6,6-bieckol are collected from fraction II by recycle-HPLC. Thus, the CPC system is useful for easy and simple isolation of phlorotannins from E. cava.

Keywords

Centrifugal partition chromatography (CPC); Ecklonia cava; Phlorotannin.

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