1. Others
  2. Fluorescent Dye
  3. Mag-Indo-1 tetrapotassium salt

Mag-Indo-1 tetrapotassium salt is a cell impermeable fluorescent probe for Mg2+ detection.

For research use only. We do not sell to patients.

Mag-Indo-1 tetrapotassium salt Chemical Structure

Mag-Indo-1 tetrapotassium salt Chemical Structure

CAS No. : 132299-21-9

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Mag-Indo-1 tetrapotassium salt is a cell impermeable fluorescent probe for Mg2+ detection[1].

In Vitro

Mag-Indo-1 tetrapotassium salt can be used to determine the free Mg2+ concentration in the cytoplasmic solutions[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Calibrating solutions containing various concentrations of Mg2+ are prepared by mixing different ratios of the two stock solutions: one contains (mM) 150 KCl, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH), and the other contains 100 MgCl2, 0.1 EGTA and 5 Hepes (pH 7.2 with KOH).
2. The calibration curve is constructed using calibrating solutions containing 1 μM Mag-Indo-1 tetrapotassium salt.
3. The ‘Mg2+’ calibrating solution contained (mM): 130 KCl, 4 EDTA and 5 Hepes (pH 7.2 with KOH).
4. The relationship between the background-corrected value of the fluorescence ratio (R) and the Mg2+ concentration is fitted with the following theoretical equation.
[Mg]=K (R-Rmin)/(Rmax-R), where [Mg] is the concentration of free Mg2+ ion, Rmin is the R value at 0 [Mg2+], and Rmax is the R value at saturating Mg2+. The curve fitting gave Rmin= 0.053, Rmax= 1.57, and K= 5.1 mM.
Note: the specific curve can refer to the reference.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

594.74

Formula

C21H14K4N2O9

CAS No.
SMILES

O=C(O[K])C1=CC2=C(C=C(N2)C3=CC=C(C(OCC(O[K])=O)=C3)N(CC(O[K])=O)CC(O[K])=O)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Mag-Indo-1 tetrapotassium salt
Cat. No.:
HY-W127843
Quantity:
MCE Japan Authorized Agent: