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  3. Mag-Fura-2 tetrapotassium

Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fura-2 tetrapotassium can be used for the determination of Mg2+ and Ca2+ concentrations.

For research use only. We do not sell to patients.

Mag-Fura-2 tetrapotassium Chemical Structure

Mag-Fura-2 tetrapotassium Chemical Structure

CAS No. : 132319-57-4

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Description

Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fura-2 tetrapotassium can be used for the determination of Mg2+ and Ca2+ concentrations[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Monitoring of Ca2+ release[1]:
1. Incubate dispersed parotid acinar cells with 8 μM Mag-fura-2 tetrapotassium for 30 min at 37℃. (acinar cells for example).
2. Wash cells twice with fresh HBSS-H without BSA.
3. Precoate cell adhesive Cell-Tak on sample chambers.
4. Transfer the dye-loaded cells to the chambers and attach to the bottom.
5. Mounte the sample chambers on the stage of an inverted microscope (equipped with a 40 × objective), wash with BSA-free HBSS-H and then with Mg2+/ATP-free ICM.
6. Incubate acinar cells with Mg2+/ATP-free ICM containing 50 μg/mL saponin for 3-5 min at room temperature.
7. Wash the cells with ICM containing Mg2+ and ATP, and incubate in the complete ICM for at least 5 min to allow complete filling of the intracellular Ca2+ stores.
8. Alternately excite permeabilised cells, capture and digitise fluorescence emission at 510 nm by a digital imaging system (record the 344 nm/360 nm ratio every 20 s).
Note: ICM (intracellular-like medium) containing 125 mM KCl, 19 mM NaCl, 10 mM HEPES (pH 7.3 with KOH), 3 mM ATP, 1.4 mM MgCl2, 0.33 mM CaCl2, and 1 mM EGTA.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

586.67

Formula

C18H10K4N2O11

CAS No.
Emission (Em)

510

Excitation (Ex)

340

SMILES

O=C(C1=CN=C(C2=CC3=C(C=C(N(CC(O[K])=O)CC(O[K])=O)C(OCC(O[K])=O)=C3)O2)O1)O[K]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Product Name:
Mag-Fura-2 tetrapotassium
Cat. No.:
HY-D1702
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