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Lucifer Yellow CH dipotassium salt 

Cat. No.: HY-W127715 Purity: 99.77%
COA Handling Instructions

Lucifer Yellow CH dipotassium is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.

For research use only. We do not sell to patients.

Lucifer Yellow CH dipotassium salt Chemical Structure

Lucifer Yellow CH dipotassium salt Chemical Structure

CAS No. : 71206-95-6

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 172 In-stock
Solution
10 mM * 1 mL in DMSO USD 172 In-stock
Solid
5 mg USD 150 In-stock
10 mg USD 240 In-stock
25 mg USD 480 In-stock
50 mg USD 800 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Lucifer Yellow CH dipotassium salt:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Lucifer Yellow CH dipotassium salt

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Lucifer Yellow CH dipotassium is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively[1][2].

In Vitro

Preparation of Lucifer Yellow CH (dipotassium) working solution
1 Preparation of the stock solution
Dissolve Lucifer Yellow CH (dipotassium) in DDH2O to obtain 1 mg/mL of Lucifer Yellow CH (dipotassium).
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Preparation of Lucifer Yellow CH (dipotassium) working solution.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5% of Lucifer Yellow CH (dipotassium) working solution.
Note: Please adjust the concentration of Lucifer Yellow CH (dipotassium) working solution according to the actual situation.

Cell staining
1. Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2. Add 1 mL of Lucifer Yellow CH (dipotassium) working solution, and then incubate at room temperature for 5-30 minutes.
3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4. Wash twice with PBS, 5 minutes each time.
5. Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of Lucifer Yellow CH (dipotassium) working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

521.57

Formula

C13H9K2N5O9S2

CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

650

Excitation (Ex)

450

SMILES

O=C(NN1C(C2=CC(S(=O)(O[K])=O)=CC3=C(C(S(=O)(O[K])=O)=CC(C1=O)=C23)N)=O)NN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (159.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 5 mg/mL (9.59 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9173 mL 9.5864 mL 19.1729 mL
5 mM 0.3835 mL 1.9173 mL 3.8346 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.87%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.9173 mL 9.5864 mL 19.1729 mL 47.9322 mL
5 mM 0.3835 mL 1.9173 mL 3.8346 mL 9.5864 mL
DMSO 10 mM 0.1917 mL 0.9586 mL 1.9173 mL 4.7932 mL
15 mM 0.1278 mL 0.6391 mL 1.2782 mL 3.1955 mL
20 mM 0.0959 mL 0.4793 mL 0.9586 mL 2.3966 mL
25 mM 0.0767 mL 0.3835 mL 0.7669 mL 1.9173 mL
30 mM 0.0639 mL 0.3195 mL 0.6391 mL 1.5977 mL
40 mM 0.0479 mL 0.2397 mL 0.4793 mL 1.1983 mL
50 mM 0.0383 mL 0.1917 mL 0.3835 mL 0.9586 mL
60 mM 0.0320 mL 0.1598 mL 0.3195 mL 0.7989 mL
80 mM 0.0240 mL 0.1198 mL 0.2397 mL 0.5992 mL
100 mM 0.0192 mL 0.0959 mL 0.1917 mL 0.4793 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Lucifer Yellow CH dipotassium salt
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