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  2. Fluorescent Dye Reactive Oxygen Species
  3. HKSOX-1m (5/6-mixture) (hydrobromide)

HKSOX-1m (5/6-mixture) (hydrobromide) 

Cat. No.: HY-D1156A Purity: 97.96%
COA Handling Instructions

HKSOX-1m (5/6-mixture) hydrobromide is a O2 fluorescent probe for mitochondria-targeting (Ex/Em=509/534nm; green), exhibiting excellent selectivity and sensitivity toward O2 over a broad range of pH, strong oxidants, and abundant reductants found in cells.

For research use only. We do not sell to patients.

HKSOX-1m (5/6-mixture) (hydrobromide) Chemical Structure

HKSOX-1m (5/6-mixture) (hydrobromide) Chemical Structure

Size Price Stock Quantity
1 mg USD 375 In-stock
5 mg USD 990 In-stock
10 mg USD 1628 In-stock
25 mg USD 3256 In-stock
50 mg USD 4884 In-stock
100 mg USD 7326 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of HKSOX-1m (5/6-mixture) (hydrobromide):

Top Publications Citing Use of Products

1 Publications Citing Use of MCE HKSOX-1m (5/6-mixture) (hydrobromide)

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HKSOX-1m (5/6-mixture) hydrobromide is a O2 fluorescent probe for mitochondria-targeting (Ex/Em=509/534nm; green), exhibiting excellent selectivity and sensitivity toward O2 over a broad range of pH, strong oxidants, and abundant reductants found in cells[1].

In Vitro

HKSOX-1m (5/6-mixture) hydrobromide (10 μM) sensitively captures signals for basal and Antimycin A (1 μM)-stimulated mitochondrial O2 in differentiated human THP-1 cells, at very low laser power output (1% intensity at Ex 514 nm on Zeiss LSM 510 Meta)[1].

1. Preparation of HKSOX-1m (5/6-mixture) hydrobromide working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKSOX-1m (5/6-mixture) hydrobromide in 79 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKSOX-1m (5/6-mixture) hydrobromide working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKSOX-1m (5/6-mixture) hydrobromide working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1258.72

Formula

C49H37Br2F10N2O10PS2

Appearance

Solid

Emission (Em)

534

Excitation (Ex)

509

SMILES

O=C1OC(C(C2=C3F)=CC(F)=C3O[S](C(F)(F)F)(=O)=O)(C4=CC(C(N(CC5)CCN5CCCC[P+](C6=CC=CC=C6)(C7=CC=CC=C7)C8=CC=CC=C8)=O)=CC=C41)C(C(O2)=C9F)=CC(F)=C9O[S](C(F)(F)F)(=O)=O.O=C%10OC(C(C%11=C%12F)=CC(F)=C%12O[S](C(F)(F)F)(=O)=O)(C(C%10=C%13)=CC=C%13C(N(CC%14)CCN%14CCCC[P+](C%15=CC=CC=C%15)(C%16=CC=CC=C%16)C%17=CC=CC=C%17)=O)C(C(O%11)=C%18F)=CC(F)=C%18O[S](C(F)(F)F)(=O)=O.[Br-].Br.[Br-].Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (158.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7945 mL 3.9723 mL 7.9446 mL
5 mM 0.1589 mL 0.7945 mL 1.5889 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.55%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7945 mL 3.9723 mL 7.9446 mL 19.8614 mL
5 mM 0.1589 mL 0.7945 mL 1.5889 mL 3.9723 mL
10 mM 0.0794 mL 0.3972 mL 0.7945 mL 1.9861 mL
15 mM 0.0530 mL 0.2648 mL 0.5296 mL 1.3241 mL
20 mM 0.0397 mL 0.1986 mL 0.3972 mL 0.9931 mL
25 mM 0.0318 mL 0.1589 mL 0.3178 mL 0.7945 mL
30 mM 0.0265 mL 0.1324 mL 0.2648 mL 0.6620 mL
40 mM 0.0199 mL 0.0993 mL 0.1986 mL 0.4965 mL
50 mM 0.0159 mL 0.0794 mL 0.1589 mL 0.3972 mL
60 mM 0.0132 mL 0.0662 mL 0.1324 mL 0.3310 mL
80 mM 0.0099 mL 0.0497 mL 0.0993 mL 0.2483 mL
100 mM 0.0079 mL 0.0397 mL 0.0794 mL 0.1986 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
HKSOX-1m (5/6-mixture) (hydrobromide)
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HY-D1156A
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