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  2. Fluorescent Dye
  3. Fura Red

Fura Red is a Ca2+-sensitive fluorescent dye, which decreases in fluorescence with rising [Ca2+].

For research use only. We do not sell to patients.

Fura Red Chemical Structure

Fura Red Chemical Structure

CAS No. : 149732-62-7

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Description

Fura Red is a Ca2+-sensitive fluorescent dye, which decreases in fluorescence with rising [Ca2+][1].

In Vitro

Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Incubate the cells according to your normal protocol.
2. Suspend cells at 1x107 cells/mL in 37°C Hanks Balanced Salt Solution (HBSS) with 1 μM Fura Red, AM unless specified otherwise and 0.01% Pluronic F127, and incubate in a 37°C water bath for 30 minutes.
3. Wash cells in HEPES Buffered Saline Solution (HBSS with 1 mM CaCl2, 0.5 mM MgCl2, 0.1% BSA, 10 mM HEPES) and resuspend to 1x107 cells/mL in HEPES Buffered Saline Solution with viability dye, SYTOX Green.
4. Equilibrate cells for ≥10 minutes in a 37°C water bath.
5. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. Ratiometric analysis of Fura Red is measured by excitation by the Violet laser (406 nm) and the Green laser (532 nm). Emission is detected by two different filter sets: increases in emission are monitored off the Violet laser (630LP and 660/20 BP), while a decrease in emission are detected off the Green laser (685LP and 710/50 BP).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

944.87

Formula

C41H44N4O20S

CAS No.
Emission (Em)

652

Excitation (Ex)

471

SMILES

O=C(OCOC(C)=O)CN(C1=CC(OC(/C=C(C(N2)=O)/NC2=S)=C3)=C3C=C1OCCOC(C=C(C)C=C4)=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Fura Red
Cat. No.:
HY-104056
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