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  3. BODIPY FL C5-Ceramide

The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye. BODIPY Fl C5-Ceramide is a Golgi-specific green fluorescent dye, which can visualise individual cells. Ex/Em= 505 nm/512 nm.

For research use only. We do not sell to patients.

BODIPY FL C5-Ceramide Chemical Structure

BODIPY FL C5-Ceramide Chemical Structure

CAS No. : 133867-53-5

Size Price Stock Quantity
1 mg USD 150 In-stock
5 mg USD 380 In-stock
10 mg   Get quote  
50 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

The Golgi apparatus is composed of flattened vesicles superimposed on each other by unit membranes. The flattened vesicles are round with expanded and perforated edges. The Golgi fluorescent probe is a BODIPY-labeled ceramide derivative, the synthesis of which occurs in the endoplasmic reticulum and can then be transported to the Golgi via ceramide transport protein (CERT) or vesicular translocation, allowing specific labeling of the dye[1]. BODIPY Fl C5-Ceramide is a Golgi-specific green fluorescent dye, which can visualise individual cells[2]. Ex/Em= 505 nm/512 nm.

In Vitro

General Protocol
1 Preparation of Golgi working solution
1.1 Preparation of the stock solution
Dissolve Golgi in DMSO to obtain 5 mM of Golgi.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Golgi working solution
Dilute the stock solution in HBSS to obtain 1-10 μM of Golgi working solution.
Note: Please adjust the concentration of Golgi working solution according to the actual situation. 2 Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 20-30 minutes.
c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 20-30 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

Storage
-20℃ 1 year.Protect from light.

Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of Golgi 1-43 working solution according to the actual situation.
3. when the cell culture solution is removed with poor results, the cells can be washed with an appropriate amount of Hanks balanced salt solution.
4. This product is for R&D use only, not for drug, household, or other uses.
5. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

601.62

Formula

C34H54BF2N3O3

CAS No.
Appearance

Solid

Color

Brown to red

Emission (Em)

511

Excitation (Ex)

505

SMILES

O=C(CCCCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])N[C@H]([C@@H](/C=C/CCCCCCCCCCCCC)O)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (83.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6622 mL 8.3109 mL 16.6218 mL
5 mM 0.3324 mL 1.6622 mL 3.3244 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.65%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6622 mL 8.3109 mL 16.6218 mL 41.5545 mL
5 mM 0.3324 mL 1.6622 mL 3.3244 mL 8.3109 mL
10 mM 0.1662 mL 0.8311 mL 1.6622 mL 4.1554 mL
15 mM 0.1108 mL 0.5541 mL 1.1081 mL 2.7703 mL
20 mM 0.0831 mL 0.4155 mL 0.8311 mL 2.0777 mL
25 mM 0.0665 mL 0.3324 mL 0.6649 mL 1.6622 mL
30 mM 0.0554 mL 0.2770 mL 0.5541 mL 1.3851 mL
40 mM 0.0416 mL 0.2078 mL 0.4155 mL 1.0389 mL
50 mM 0.0332 mL 0.1662 mL 0.3324 mL 0.8311 mL
60 mM 0.0277 mL 0.1385 mL 0.2770 mL 0.6926 mL
80 mM 0.0208 mL 0.1039 mL 0.2078 mL 0.5194 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
BODIPY FL C5-Ceramide
Cat. No.:
HY-D1612
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