2. Induced Disease Models Products Immunology/Inflammation Metabolic Enzyme/Protease
  3. Immunology and Inflammatory Disease Models Digestive System Disease Models NO Synthase Endogenous Metabolite
  4. Pancreatitis Models Pancreatic Disease Models
  5. L-Arginine hydrochloride

L-Arginine hydrochloride  (Synonyms: (S)-(+)-Arginine hydrochloride)

Cat. No.: HY-N0455A Purity: ≥98.0%
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L-Arginine ((S)-(+)-Arginine) is the substrate for the endothelial nitric oxide synthase (eNOS) to generate NO. L-Arginine is transported into vascular smooth muscle cells by the cationic amino acid transporter family of proteins where it is metabolized to nitric oxide (NO), polyamines, or L-proline. L-Arginine is a potent vasodilator, and can be used to induce experimental acute pancreatitis.

For research use only. We do not sell to patients.

L-Arginine hydrochloride Chemical Structure

L-Arginine hydrochloride Chemical Structure

CAS No. : 1119-34-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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10 mM * 1 mL in Water USD 55 In-stock
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L-Arginine hydrochloride Related Antibodies

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Description

L-Arginine ((S)-(+)-Arginine) is the substrate for the endothelial nitric oxide synthase (eNOS) to generate NO. L-Arginine is transported into vascular smooth muscle cells by the cationic amino acid transporter family of proteins where it is metabolized to nitric oxide (NO), polyamines, or L-proline. L-Arginine is a potent vasodilator, and can be used to induce experimental acute pancreatitis[1][2][3][4][5].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

In Vitro

Arginine is an α-amino acid. The L-form is one of the 20 most common natural amino acids. At the level of molecular genetics, in the structure of the messenger ribonucleic acid mRNA, CGU, CGC, CGA, CGG, AGA, and AGG, are the triplets of nucleotide bases or codons that code for arginine during protein synthesis. In mammals, arginine is classified as a semiessential or conditionally essential amino acid, depending on the developmental stage and health status of the individual. L-Arginine is associated with a decrease in cardiac index while stroke index is maintained in patients with severe sepsis. Resolution of shock at 72 hours is achieved by 40% and 24% of the patients in the L-Arginine and placebo cohorts, respectively. L-Arginine (450 mg/kg during a 15-minute period) amplifies and sustains the hyperemia (38%) and increases absolute brain blood flow after eNOS upregulation by chronic simvastatin treatment (2 mg/kg subcutaneously, daily for 14 days) in SV-129 mice.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L-Arginine hydrochloride Related Antibodies
In Vivo

Induction of experimental acute pancreatitis[4][5]
Background
L-Arginine is a substrate for endothelial nitric oxide synthase (eNOS) to produce NO, which can be metabolized into nitric oxide (NO), polyamines or L-proline, stimulating inflammatory responses. L-Arginine can also selectively destroy pancreatic acinar cells, leading to acute necrotizing pancreatitis.
Specific Mmodeling Methods
Rat[4]: male • Wistar albino rats • 150 g
Administration: 250 mg, 500 mg, 750 mg every 100 g body weight (L-Arginine for example; HY-N0455) • ip for single dose • starved for 15 hours
Mice[5]: male • ICT mouse • 4-week-old • 25-30 g
Administration: total 450 mg/100 g body weight • ip for 2 doses, with 1 hr interval • sacrificed at 72 hours
Note
1. 当剂量超过 500 mg/100 g 体重时，将导致大部分大鼠在注射后数小时内死亡；当接受剂量低于 250 mg 时，诱导出现的胰腺病的症状会较轻[4]。
2. 文献中溶解方法参考[5]：将 L-Arginine 粉末溶解于 0.9% 生理盐水中，用 5 N 盐酸调节 pH 至 7，制得 L-Arginine 溶液。
Modeling Indicators
Appearance changes: weight loss, lower activity, softer hair
Tissue morphology: pancreatic edema, damage, atrophy, granulation tissue contraction and chalky spots (location of fat necrosis).
Cellular changes: basophils decrease and vesicles, cell pyknosis, nuclear fragmentation. Pancreatic albino cells disappear and die, and pancreatic tissue is replaced by monocytes and fibroblasts.
Molecular changes: serum amylase levels increase, and fat necrosis is obvious.
Correlated Product(s)
Opposite Product(s) Curcumin (HY-N0005)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

210.66

Formula

C6H15ClN4O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCNC(N)=N)C(O)=O.[H]Cl
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (474.70 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7470 mL 23.7349 mL 47.4699 mL
5 mM 0.9494 mL 4.7470 mL 9.4940 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (474.70 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.85%

COA (244 KB) HNMR (175 KB) MS (279 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.7470 mL 23.7349 mL 47.4699 mL 118.6746 mL
5 mM 0.9494 mL 4.7470 mL 9.4940 mL 23.7349 mL
10 mM 0.4747 mL 2.3735 mL 4.7470 mL 11.8675 mL
15 mM 0.3165 mL 1.5823 mL 3.1647 mL 7.9116 mL
20 mM 0.2373 mL 1.1867 mL 2.3735 mL 5.9337 mL
25 mM 0.1899 mL 0.9494 mL 1.8988 mL 4.7470 mL
30 mM 0.1582 mL 0.7912 mL 1.5823 mL 3.9558 mL
40 mM 0.1187 mL 0.5934 mL 1.1867 mL 2.9669 mL
50 mM 0.0949 mL 0.4747 mL 0.9494 mL 2.3735 mL
60 mM 0.0791 mL 0.3956 mL 0.7912 mL 1.9779 mL
80 mM 0.0593 mL 0.2967 mL 0.5934 mL 1.4834 mL
100 mM 0.0475 mL 0.2373 mL 0.4747 mL 1.1867 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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L-Arginine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-Arginine1119-34-2(S)-(+)-ArginineNO SynthaseEndogenous MetaboliteNitric oxide synthasesNOSNOvascularsmoothmuscletransporterInhibitorinhibitorinhibit

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